The cytosolic invertase NI6 affects vegetative growth, flowering, fruit set, and yield in tomato
Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble and insoluble invertases have been largely inves...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/download/articulo/2021coluccioleskow.pdf LINK AL EDITOR |
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| 005 | 20250812135036.0 | ||
| 008 | 220812t2021 xxkdo|||o|||| 00| 0 eng d | ||
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| 022 | |a 0022-0957 | ||
| 024 | |a 10.1093/jxb/eraa594 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 1 | 0 | |a The cytosolic invertase NI6 affects vegetative growth, flowering, fruit set, and yield in tomato |
| 520 | |a Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble and insoluble invertases have been largely investigated, studies on the role of neutral invertases (A/N-INV) have lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with a previously reported quantitative trait locus (QTL) largely affecting primary carbon metabolism in tomato. Of the eight A/N-INV genes identified in the tomato genome, NI6 mRNA is present in all organs, but its expression was higher in sink tissues (mainly roots and fruits). A NI6-GFP fusion protein localized to the cytosol of mesophyll cells. Tomato NI6- silenced plants showed impaired growth phenotype, delayed flowering and a dramatic reduction in fruit set. Global gene expression and metabolite profile analyses of these plants revealed that NI6 is not only essential for sugar metabolism, but also plays a signaling role in stress adaptation. We also identified major hubs, whose expression patterns were greatly affected by NI6 silencing; these hubs were within the signaling cascade that coordinates carbohydrate metabolism with growth and development in tomato. | ||
| 650 | |2 Agrovoc |9 26 | ||
| 653 | |a CARBON PARTITIONING | ||
| 653 | |a CYTOSOLIC INVERTASE | ||
| 653 | |a SIGNALING | ||
| 653 | |a SUCROSE METABOLISM | ||
| 653 | |a TOMATO | ||
| 700 | 1 | |a Coluccio Leskow, Carla |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Buenos Aires, Argentina. |u CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Argentina. |9 73980 | |
| 700 | 1 | |a Conte, Mariana |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Buenos Aires, Argentina. |u CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Argentina. |9 70031 | |
| 700 | 1 | |a Del Pozo, Talia |u Universidad Mayor. Escuela de Agronomía. Centro Tecnológico de Recursos Vegetales. Huechuraba, Santiago, Chile. |9 73981 | |
| 700 | 1 | |9 68277 |a Bermúdez, Luisa |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Buenos Aires, Argentina. |u CONICE - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Argentina. |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética. Buenos Aires, Argentina. | |
| 700 | 1 | |a Silvestre Lira, Bruno |u Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica. São Paulo, SP, Brazil. |9 73982 | |
| 700 | 1 | |a Gramegna, Giovanna |u Universidade de São Paulo. Departamento de Botânica. Instituto de Biociências. São Paulo, SP, Brazil. |9 73983 | |
| 700 | 1 | |a Baroli, Irene |u Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA). Buenos Aires, Argentina. |u CONICET - Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA). Buenos Aires, Argentina. |9 73984 | |
| 700 | 1 | |9 11158 |a Carrari, Fernando |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética. Buenos Aires, Argentina. |u Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, CONICET-UBA). Buenos Aires, Argentina. |u CONICET - Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, CONICET-UBA). Buenos Aires, Argentina. | |
| 773 | 0 | |t Journal of experimental botany |w (AR-BaUFA)SECS000114 |g Vol.72, no.7 (2021), p.2525-2543, grafs., tbls., fot. | |
| 856 | |f 2021coluccioleskow |i en internet |q application/pdf |u http://ri.agro.uba.ar/files/download/articulo/2021coluccioleskow.pdf |x ARTI202206 | ||
| 856 | |u https://academic.oup.com |z LINK AL EDITOR | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||