In vitro evaluation of preabsorptive events of dispersed supramolecular aggregates by dynamic light scattering : Oral multivitamins as a model case
|Main Authors:||Manzo, Rubén H., Jiménez Kairuz, Álvaro, Palena, María C.|
Latin American Journal of Pharmacy
vol. 31, no. 1
DYNAMIC LIGHT SCATTERING; IN VITRO TEST; ORAL MULTIVITAMINS; PREABSORTIVE EVENTS; SUPRAMOLECULAR AGGREGATES
Table of Contents:
- In order to develop an approach to evaluate the preabsorptive behavior of supramolecular aggregate carriers, three commercial oral formulations of a mixture of lipophilic and hydrophilic vitamins dispersed in water-glycerin-polysorbate-80 (PS-80) vehicle were subjected to dynamic light scattering and electrokinetic determinations. They exhibited diffusion coefficients ranging from 4.46 .10<sup>-9</sup> to 3.22 .10<sup>-8</sup> cm<sup>2</sup>/s. To mimic in vivo environment the formulations were progressively diluted with water and simulated gastric and intestinal fluids until a ratio 1:10. Hydrodynamic diameters (d<sub>H</sub>) at 37 °C and electrokinetic potentials of each formulation remained almost unchanged in the three assayed conditions. However, the average d<sub>H</sub> were 1.00, 1.36 and 1.63 times higher than a reference dispersion of PS-80 revealing significant differences among formulations. Concentration of PS-80 in diluted samples is about 10<sup>3</sup> times above its critical micellar concentration. Therefore, loaded core/shell micelles appears to be robust drug carriers that will interact with the gastrointestinal membranes upon administration. The observed differences in size and/or shape would be related to the biopharmaceutical performance of multisource products. Besides, the developed approach could be used in developing and control stages of such pharmaceutical systems.
- Colegio de Farmacéuticos de la Provincia de Buenos Aires